2020. 3. 2. 15:36ㆍ카테고리 없음
Tailorable cationic chitosan/PLGA nanoparticles (CPNP) were used for the delivery of an antisense 2′- O-methyl-RNA (2OMR) directed against RNA template of human telomerase. Here, we describe the influence of the chitosan content on binding efficiency, complex stability, uptake in different human lung cell types and finally demonstrate the efficacy of this nanoplex system.CPNPs were prepared by the emulsion-solvent evaporation method using different amounts of chitosan and purified by preparative size exclusion chromatography. The characterization by photon correlation spectroscopy and zeta potential measurements showed a small increase in size and an increase of zeta potential with increasing amounts of chitosan. Binding efficiency and complex stability with 2OMR was high in water and correlated well with the chitosan content of particles but was weak in physiologically relevant media (PBS and RPMI cell culture medium). However, flow cytometry analysis showed that the uptake of 2OMR into A549 lung cancer cells was considerably higher in combination with nanoparticles and dependent on the amount of chitosan when compared to 2OMR alone. Confocal laser scanning microscopy revealed that the uptake into A549 cells is mediated via complexes of 2OMR and chitosan/PLGA nanoparticles despite the weak binding in cell culture medium. The nanoparticles were well tolerated and efficient in inhibiting telomerase activity.
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Bidding Increment Error: There was an error in the system that put all 'next bid' numbers at $5 above the current bid. If you receive an error that says 'THIS BID IS OFF INCREMENT', please try increasing your bid in $5 increments until it works. (Bids above $100 should be in $25 increments, bids above $500 in $50 increments)Thank-youAll bidders residing outside of the United States or Canada are required to submit via wire transfer a qualifying deposit of 25% of their total expected purchases.
Deposit must be received and confirmed prior to start of auction, and can take up to 48 hours to clear. Please register at least 2 business days prior to sale to allow sufficient time to process deposit. Please contact or with Maynards Industries for wire transfer information.BidSpotter Customer Service Support DepartmentTo reach a member of our qualified support staff regarding information or questions pertaining to the bidding process; please reach us by phone or email. Available Monday – Friday, 8:00am – 12:00am ET.
Email:. Office: 253-858-6777.Terms and Conditions. AUCTIONEER DISCLAIMER and BIDDER ACCEPTANCE for ALL ON LINE and WEBCAST ELECTRONIC BIDDING: AUCTIONEER DOES NOT WARRANT THAT THE FUNCTIONS, FEATURES OR CONTENT CONTAINED IN THE ONLINE OR WEBCAST BIDDING PLATFORM OR SOFTWARE, INCLUDING ANY THIRD-PARTY SOFTWARE, PRODUCTS OR OTHER MATERIALS USED IN CONNECTION WITH THE WEBSITE, WILL BE TIMELY, SECURE, UNINTERRUPTED OR ERROR-FREE, OR THAT DEFECTS WILL BE CORRECTED. AUCTIONEER RESERVES THE RIGHT TO ACCEPT AND OR REJECT AND OR REMOVE ANY ELECTRONIC BIDS FOR ANY REASON AT ANY TIME IN ITS SOLE DISCRETION. BIDDER AGREES ALL OF ITS ELECTRONIC BIDS ARE BINDING REGARDLESS OF ORDER AND OR TIME IN WHICH THEY ARE ACCEPTED OR REJECTED.The following, as amended by any posted notices and/or oral announcements during the auction sale, constitutes the entire terms and conditions on which property listed in the auction sale catalogue shall be offered for sale or sold by Maynards, and any consignor of such property for whom Maynards acts as agent. Maynards strongly recommends to all bidders to thoroughly inspect all items before the auction on which bidder intends to bid. Under these Terms and Conditions, the term “Bidder” and “Purchaser” shall be used interchangeable and be one of the same.1.
Bidder acknowledges that an auction site is a potentially dangerous place. Flammable, noxious, corrosive and pressurized substances may be present, heavy equipment may be in operation, and electric circuits may be live. Every person at the auction site before, during and after the auction sale, shall be deemed to be there at his/her own risk with notice of the condition of the premises, the activities on the premises, and conduct of third parties; and the bidder shall so advise his/her agents and employees. No person shall have any claim against Maynards, its consignors, agents, employees, directors or principals for any injuries sustained or for damages to or loss of personal property, which may occur from any cause whatsoever.2. As used herein, the term “Bid Price” means the price at which a lot is knocked down to the Purchaser, and the term “Purchase Price” means the aggregate of (a) the Bid Price, (b) the Buyer’s Premium and other surcharges, if any, and (c) unless Purchaser is exempt by law from the payment thereof, any Federal, Provincial, State or local sales taxes (or compensating use tax of another State), and any other applicable taxes.
Unless exemption from such taxes is established to the satisfaction of Maynards, any Purchaser claiming an exemption will be required to pay the tax to Maynards and seek a refund directly from the taxing authorities.3. A sale to the highest Bidder shall be complete upon the auctioneer’s call, with the highest Bidder purchasing the offered lot, in accordance with all the conditions set forth herein, including Purchaser (a) assuming full risk of loss and responsibility thereof, see Point 8 below, (b) immediately making a deposit of not less than twenty-five percent (25%) of the Purchase Price, and (c) paying to Maynards the balance of the Purchase Price in full within forty-eight (48) hours after the auction sale for all lots purchased. No lot may be removed until the Purchase Price is paid in full for all lots purchased. To prevent misdelivery and inconvenience in settlement of a purchase, no lot may be transferred prior to removal by the Purchaser.4.
Bidding Increment Error: There was an error in the system that put all 'next bid' numbers at $5 above the current bid. If you receive an error that says 'THIS BID IS OFF INCREMENT', please try increasing your bid in $5 increments until it works. (Bids above $100 should be in $25 increments, bids above $500 in $50 increments)Thank-youAll bidders residing outside of the United States or Canada are required to submit via wire transfer a qualifying deposit of 25% of their total expected purchases. Deposit must be received and confirmed prior to start of auction, and can take up to 48 hours to clear. Please register at least 2 business days prior to sale to allow sufficient time to process deposit.
Please contact or with Maynards Industries for wire transfer information.BidSpotter Customer Service Support DepartmentTo reach a member of our qualified support staff regarding information or questions pertaining to the bidding process; please reach us by phone or email. Available Monday – Friday, 8:00am – 12:00am ET. Email:. Office: 253-858-6777.Terms and Conditions. AUCTIONEER DISCLAIMER and BIDDER ACCEPTANCE for ALL ON LINE and WEBCAST ELECTRONIC BIDDING: AUCTIONEER DOES NOT WARRANT THAT THE FUNCTIONS, FEATURES OR CONTENT CONTAINED IN THE ONLINE OR WEBCAST BIDDING PLATFORM OR SOFTWARE, INCLUDING ANY THIRD-PARTY SOFTWARE, PRODUCTS OR OTHER MATERIALS USED IN CONNECTION WITH THE WEBSITE, WILL BE TIMELY, SECURE, UNINTERRUPTED OR ERROR-FREE, OR THAT DEFECTS WILL BE CORRECTED. AUCTIONEER RESERVES THE RIGHT TO ACCEPT AND OR REJECT AND OR REMOVE ANY ELECTRONIC BIDS FOR ANY REASON AT ANY TIME IN ITS SOLE DISCRETION.
BIDDER AGREES ALL OF ITS ELECTRONIC BIDS ARE BINDING REGARDLESS OF ORDER AND OR TIME IN WHICH THEY ARE ACCEPTED OR REJECTED.The following, as amended by any posted notices and/or oral announcements during the auction sale, constitutes the entire terms and conditions on which property listed in the auction sale catalogue shall be offered for sale or sold by Maynards, and any consignor of such property for whom Maynards acts as agent. Maynards strongly recommends to all bidders to thoroughly inspect all items before the auction on which bidder intends to bid. Under these Terms and Conditions, the term “Bidder” and “Purchaser” shall be used interchangeable and be one of the same.1. Bidder acknowledges that an auction site is a potentially dangerous place.
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Flammable, noxious, corrosive and pressurized substances may be present, heavy equipment may be in operation, and electric circuits may be live. Every person at the auction site before, during and after the auction sale, shall be deemed to be there at his/her own risk with notice of the condition of the premises, the activities on the premises, and conduct of third parties; and the bidder shall so advise his/her agents and employees.
No person shall have any claim against Maynards, its consignors, agents, employees, directors or principals for any injuries sustained or for damages to or loss of personal property, which may occur from any cause whatsoever.2. As used herein, the term “Bid Price” means the price at which a lot is knocked down to the Purchaser, and the term “Purchase Price” means the aggregate of (a) the Bid Price, (b) the Buyer’s Premium and other surcharges, if any, and (c) unless Purchaser is exempt by law from the payment thereof, any Federal, Provincial, State or local sales taxes (or compensating use tax of another State), and any other applicable taxes. Unless exemption from such taxes is established to the satisfaction of Maynards, any Purchaser claiming an exemption will be required to pay the tax to Maynards and seek a refund directly from the taxing authorities.3.
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A sale to the highest Bidder shall be complete upon the auctioneer’s call, with the highest Bidder purchasing the offered lot, in accordance with all the conditions set forth herein, including Purchaser (a) assuming full risk of loss and responsibility thereof, see Point 8 below, (b) immediately making a deposit of not less than twenty-five percent (25%) of the Purchase Price, and (c) paying to Maynards the balance of the Purchase Price in full within forty-eight (48) hours after the auction sale for all lots purchased. No lot may be removed until the Purchase Price is paid in full for all lots purchased. To prevent misdelivery and inconvenience in settlement of a purchase, no lot may be transferred prior to removal by the Purchaser.4.